|
|
|
| Registros recuperados: 40 | |
|
| |
|
|
| Suda,Cecilia N. K.; Buckeridge,Marcos S.; Giorgini,Jarbas F.. |
| Activities of cell wall hydrolases of Euphorbia heterophylla L. (wild poinsettia) endosperm were investigated during pre- and post-emergence periods, defined as the time interval before and after 2.2 days from the start of imbibition, respectively. The activities of endo-beta-mannanase and beta-mannosidase are higher over the pre-emergence when compared to the post-emergence period and they may be involved in the process of germination in E. heterophylla. On the other hand, the activities of beta-galactosidase, beta-glucosidase, alpha-xylosidase, beta-xylosidase and glucanases, which hydrolyse CMC, xyloglucans from Hymenaea courbaril or Copaifera langsdorffii, xylan, Avicel and lichenan, are higher over the post-emergence period. Activity on laminarin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledon expansion; Endosperm; Endoglucanase; Endomannanase; Euphorbiaceae; Xylanase. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000300002 |
| |
|
|
| Heck,Júlio X.; Hertz,Plinho F.; Ayub,Marco A.Z.. |
| In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max) protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solid-State cultivation; Cellulase; Xylanase; Soybean residue; Amazon biodiversity. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300005 |
| |
|
|
| Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying. |
| A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced. The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11 of glycosyl hydrolases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanase; Gene cloning; Fusariumsp. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049 |
| |
|
|
| Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. |
| ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 |
| |
|
|
| Ahmed,Sibtain; Imdad,Syeda Sana; Jamil,Amer. |
| Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T.... |
| Tipo: Journal article |
Palavras-chave: C. thermophilum; Kinetics; T. harzianum; Xylanase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003 |
| |
|
|
| Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP. |
| Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p>0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008 |
| |
|
|
| Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC. |
| ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p<0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300 |
| |
|
|
| Zanelato,A. I.; Shiota,V. M.; Gomes,E.; Silva,R. da; Thoméo,J. C.. |
| This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3busing a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50ºC), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7:3, 50ºC temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Xylanase; Myceliophthora sp.; Packed bed; Solid state-fermentation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400038 |
| |
|
| |
|
|
| Martínez Aispuro, José Alfredo. |
| El presente trabajo tuvo como objetivo reducir y/u optimizar la concentración de algunos nutrimentos en las dietas de cerdos de engorda que puede resultar benéfica en la formulación de raciones. Se realizaron 3 experimentos para evaluar el nivel de lisina, el uso de alcaloides y xilanasas en dietas para cerdos de engorda. En el experimento 1 se determinaron los niveles óptimos biológicos (NOB) de lisina total para cerdos en las etapas de crecimiento y finalización en el comportamiento productivo, las características de la canal, y la concentración de urea en plasma. El NOB para ganancia diaria de peso (GDP), conversión alimenticia, y ganancia de carne magra fue 0.88% de lisina total; mientras que el NOB para consumo de alimento fue 0.961% de lisina total.... |
|
Palavras-chave: Lisina; Alcaloides; Dietas con baja proteína; Xilanasas; Energía; Proteína; Lysine; Alkaloids; Low protein diets; Xylanase; Energy; Protein; Ganadería; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2159 |
| |
|
|
| Neumann,Mikael; Leão,Guilherme Fernando Mattos; Horst,Egon Henrique; Stuani,Osmair Flavio; Sangali,Cleiton Pagliari; Castilho,Ricardo. |
| ABSTRACT The objective of this study was to evaluate the effects of supplementation of two different enzymatic complexes, fibrolytic (NSPases) or amylolytic (EXP3066) enzymes, on growth performance, digestibility, behavior, and carcass characteristics of feedlot cattle. Thirty-six ½ Angus yearling bulls with an average initial weight of 391±5.0 kg were used in this experiment blocked by weight in a completely randomized design divided in three treatments: control, NSPases, and EXP3066. The basal diet was composed of 85% whole corn grain and 15% nucleus. NSPases increased average daily gain on days 63 and 84, and gain:feed ratio on days 42, 63, and 84. Total carcass weight and carcass daily gain were improved by 4.8% and up to 6.0% with EXP3066 and NSPases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Corn; High-energy diet; Non-starch polysaccharides; Xylanase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100611 |
| |
|
|
| Santana,Yânez André Gomes; Vasconcelos,Vânia Rodrigues; Alves,Arnaud Azevêdo; Silva,Suzana Coimbra de Moura Lustosa e; Garcez,Bruno Spindola. |
| ABSTRACT The effect of treatment with xylanase and β-glucanase was evaluated for gas production and the ruminal degradation of nutrients from the hay of Tifton 85 grass and the stubble of maize, sorghum, peanut, sunflower and sesame crops. Two commercial fibrolytic enzymes were used (Dyadic xylanase PLUS - Xylanase; BrewZyme LP-β-glucanase), added to the hay at doses of 7.5 units of endoglucanase and 0.46 units of xylanase per 500 mg/gDM, for the cellulase and xylanase products respectively. The chemical composition of the hay was determined for no enzyme application and 24 hours after enzyme treatment, and the in vitro gas production and in situ microbial degradation was estimated for dry matter, organic matter, neutral detergent fibre and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis hypogaea; Β-glucanase; Helianthus annuus; Sesamum indicum; Sorghum bicolor; Xylanase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100167 |
| |
|
|
| Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F.. |
| Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Trichoderma; Xylan; Xylanase. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003 |
| |
|
|
| Yi,Xiuli; Shi,Yan; Xu,Hui; Li,Wei; Xie,Jie; Yu,Rongqing; Zhu,Jun; Cao,Yi; Qiao,Dairong. |
| The analysis of individual gene product should enable to clarify the role of a particular enzyme in a complex xylanase system of A. niger. The two genes encoding precursors of co-produced endo-1,4-β-D-xylanases, xynA1 and xynB, were isolated from Aspergillus niger SCTCC 400264 (SCTCC, China) by using RT-PCR technique and then successfully expressed in Escherichia coli BL21. The nucleotide sequences of the xynA1 and xynB genes revealed that they were only 52.5% homology to each other. Characterization of the recombinant enzymes revealed the different properties: the specific activity of recombinant XYNA1 was 16.58 U/mg compared to 1201.7 U/mg for recombinant XYNB; The optimum temperature and pH of the recombinant XYNA1 were 35 ºC and 3.0, respectively,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Xylanase; Prokaryotic expression; Enzymatic characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300030 |
| |
|
| |
|
|
| Joshi,Chetna; Khare,Sunil Kumar. |
| Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Induction; Isoforms; Xylanase; Biobleaching. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003 |
| |
|
| |
|
| |
| Registros recuperados: 40 | |
|
|
|
